|Analysis of DNA methylation using bisulphite sequencing - METHODS.info choice!|
|Discuss this protocol
Bisulphite treatment by Dr. Ushijima (Modified by Alexei Gratchev)
This protocol for bisulphite treatment was kindly sent to me by Dr. Toshikazu Ushijima. I was intrigued, since in this protocol you can use relatively high amounts of DNA and there is no need of generating agarose beads the most critical step in our old protocol. Unfortunately I was not able to test it myself yet, but my colleague, Dr. Natalia Kisseljova from the Russian Cancer Research Centre tested it. She was really impressed by the results, since this protocol leads to almost 100% conversion of nonmethylated cytosines. Even the region of DNA Dr. Kisseljova considers the most difficult, was converted the best when bisulphite treatment protocol Dr. Ushijima suggested. The usage of up to 2 mkg of DNA makes it much easier to amplify your target sequence.
|\METHODS\DNA methylation analysis\Analysis of methylation using bisulphite sequencing|
|© Copyright 1999-2006 Alexei Gratchev. All rights reserved.|