|First strand cDNA synthesis with Ready-To-Go Beads (Amersham)|
This cDNA synthesis system simplifies your work dramatically. All reaction components are premixed and lyophylised. You have to add your RNA and (for Your-Prime beads) the primer. Another advantage of the system is a little number of pipetting steps required, and therefore reduced risk of RNase contamination and RNA degradation. Company control of the product provides you with standard reaction conditions. The kit can be stored at RT. The only disadvantage which I observed is a bit lower reaction efficiency in comparison to the conventional reaction when you have to add all your components separately.
You can control your cDNA preparaion for reaction efficiency and genomic DNA contamination using protocol provided here.
|\METHODS\cDNA\Ready to Go Beads (Amersham)|
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